cDNA cloning and analysis of tissue-specific gene expression of rat urocortin II = 랫트 유로코틴2의 조직특이적 발현의 cDNA클론닝 및 분석

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Title
cDNA cloning and analysis of tissue-specific gene expression of rat urocortin II = 랫트 유로코틴2의 조직특이적 발현의 cDNA클론닝 및 분석
Author(s)
Jung Il Chea; Seong Kyu Joo; Myung Kyu Lee; J H Park; J H Yoon; J H Shim; D S Lee
Bibliographic Citation
Molecular Biology, vol. 43, no. 1, pp. 82-87
Publication Year
2009
Abstract
The corticotropin-releasing hormone (CRH) family of neuropeptides includes CRH (a 41-amino acid hypothalamic peptide) and urocortin. Corticotropin- releasing factor (CRF), a peptide first isolated from mammalian, plays an important role in the regulation of the pituitary-adrenal axis, and in endocrine, autonomic, immune and behavioral responses to stress. In this study, we cloned rat urocortin II (UCN II) cDNA from rat mid-brain by RT-PCR. The rat UCN II clone contained an open reading frame (ORF) 109 amino acids which shared 90% and 63% homology with mouse and human homologues, respectively. The expression of UCN II mRNA is mainly distributed in bone marrow, ovary, uterus, hypophysis, adrenal gland, and skin. In this study, rat recombinant UCN was expressed in E. coli and purified in active form. Furthermore, purified recombinant UCN II protein specifically binds to CRF receptor 2 in rat ROS 17 / 2.8 and GH3 cells by flow-cytometry analysis. UCN II cDNA clone obtained in this study will be useful for further investigation on behavioral responses to stress in rats.
Keyword
recombinant proteinsurocortin IIFACS
ISSN
0026-8933
Publisher
Springer
DOI
http://dx.doi.org/10.1134/S0026893309010117
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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