Obovatol isolated from Magnolia obovata enhances pentobarbital-induced sleeping time: Possible involvement of GABA(A) receptors/chloride channel activation = 오보바톨의 수면 개선 효과

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Title
Obovatol isolated from Magnolia obovata enhances pentobarbital-induced sleeping time: Possible involvement of GABA(A) receptors/chloride channel activation = 오보바톨의 수면 개선 효과
Author(s)
H Ma; Y J Jo; Y Ma; J T Hong; Byoung-Mog Kwon; K W Oh
Bibliographic Citation
Phytomedicine, vol. 16, no. 4, pp. 308-313
Publication Year
2009
Abstract
This study aimed to investigate the effects of obovatol isolated from Magnolia obovata on pentobarbital-induced sleeping behaviors and to determine whether these effects were mediated by GABAA receptors/chloride channel activation, using a western blot technique and Cl- sensitive fluorescence probe. GABAA receptors subunits expression and chloride influx were investigated in cultured cerebellar granule cells. Obovatol (0.05, 0.1, and 0.2 mg/kg) prolonged the sleeping time induced by pentobarbital (42 mg/kg). In addition, obovatol (20 and 50 μM) significantly increased Cl- influx in the primary cultured cerebellar granule cells. Moreover, obovatol increased the expression of GABAA receptor α-, β-, and γ-subunits. However, it had no effect on the abundance of the expression of glutamic acid decarboxylase (GAD), suggesting that obovatol might not activate GAD. These results suggest that obovatol potentiates pentobarbital-induced sleeping time through the GABAA receptors/chloride channel activation.
Keyword
Chloride influxGABAA receptorsGlutamic acid decarboxylase (GAD)Magnolia obovataObovatolPentobarbitalSleep
ISSN
0944-7113
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.phymed.2008.12.007
Type
Article
Appears in Collections:
Division of Biomedical Research > Genome Editing Research Center > 1. Journal Articles
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