Detection of biomolecular binding through enhancement of localized surface plasmon resonance (LSPR) by gold nanoparticles

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Title
Detection of biomolecular binding through enhancement of localized surface plasmon resonance (LSPR) by gold nanoparticles
Author(s)
H M KIm; S M Jin; S K Lee; Min-Gon Kim; Yong Beom Shin
Bibliographic Citation
Sensors, vol. 9, no. 4, pp. 2334-2344
Publication Year
2009
Abstract
To amplify the difference in localized surface plasmon resonance (LSPR) spectra of gold nano-islands due to intermolecular binding events, gold nanoparticles were used. LSPR-based optical biosensors consisting of gold nano-islands were readily made on glass substrates using evaporation and heat treatment. Streptavidin (STA) and biotinylated bovine serum albumin (Bio-BSA) were chosen as the model receptor and the model analyte, respectively, to demonstrate the effectiveness of this detection method. Using this model system, we were able to enhance the sensitivity in monitoring the binding of Bio- BSA to gold nano-island surfaces functionalized with STA through the addition of gold nanoparticle-STA conjugates. In addition, SU-8 well chips with gold nano-island surfaces were fabricated through a conventional UV patterning method and were then utilized for image detection using the attenuated total reflection mode. These results suggest that the gold nano-island well chip may have the potential to be used for multiple and simultaneous detection of various bio-substances.
Keyword
attenuated total reflection (ATR)gold nano-islandgold nanoparticlelocalized surface plasmon resonance (LSPR)well chip
ISSN
1424-8220
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/s90402334
Type
Article
Appears in Collections:
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
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