Rengyolone inhibits apoptosis via etoposide-induced caspase downregulation

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Title
Rengyolone inhibits apoptosis via etoposide-induced caspase downregulation
Author(s)
Jin Hee Kim; C H Lee
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 19, no. 3, pp. 286-290
Publication Year
2009
Abstract
In the course of screening for substances inhibiting apoptosis of U937 human leukemia cells induced by etoposide (10 μg/ml), Forsythiae fructus, which showed a high level of inhibition, was selected. The regulating compounds were purified from the ethyl acetate extract by silica gel column chromatography and HPLC. The active substance was purified and identified as rengyolone by spectroscopic methods. This compound showed inhibitory activity on caspase-3 induction, a major protease of the apoptosis cascade, with an IC50 value of 38.96 μM after 8 h of etoposide treatment in U937 cells. The expression level of caspase-3 and poly(ADP-ribose) polymerase (PARP) were dose-dependently inhibited by the compound, suggesting that rengyolone inhibits etoposide-induced apoptosis via downregulation of caspases.
Keyword
apoptosisrengyolone
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.4014/jmb.0804.253
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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