Cited 4 time in
- Title
- Rengyolone inhibits apoptosis via etoposide-induced caspase downregulation
- Author(s)
- Jin Hee Kim; C H Lee
- Bibliographic Citation
- Journal of Microbiology and Biotechnology, vol. 19, no. 3, pp. 286-290
- Publication Year
- 2009
- Abstract
- In the course of screening for substances inhibiting apoptosis of U937 human leukemia cells induced by etoposide (10 μg/ml), Forsythiae fructus, which showed a high level of inhibition, was selected. The regulating compounds were purified from the ethyl acetate extract by silica gel column chromatography and HPLC. The active substance was purified and identified as rengyolone by spectroscopic methods. This compound showed inhibitory activity on caspase-3 induction, a major protease of the apoptosis cascade, with an IC50 value of 38.96 μM after 8 h of etoposide treatment in U937 cells. The expression level of caspase-3 and poly(ADP-ribose) polymerase (PARP) were dose-dependently inhibited by the compound, suggesting that rengyolone inhibits etoposide-induced apoptosis via downregulation of caspases.
- Keyword
- apoptosisrengyolone
- ISSN
- 1017-7825
- Publisher
- Korea Soc-Assoc-Inst
- DOI
- http://dx.doi.org/10.4014/jmb.0804.253
- Type
- Article
- Appears in Collections:
- 1. Journal Articles > Journal Articles
- Files in This Item:
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