Backbone resonance assignment of a proteolysis-resistant fragment in the oxygen-dependent degradation domain of the hypoxia inducible factor 1alpha

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Title
Backbone resonance assignment of a proteolysis-resistant fragment in the oxygen-dependent degradation domain of the hypoxia inducible factor 1alpha
Author(s)
D H Kim; Si-Hyung Lee; Seung-Wook Chi; Ki Hoon Nam; Kyou Hoon Han
Bibliographic Citation
Molecules and Cells, vol. 27, no. 4, pp. 493-496
Publication Year
2009
Abstract
Hypoxia-inducible factor 1α (HIF1α) is a transcription factor that plays a key role in the adaptation of cells to low oxygen stress and oxygen homeostasis. The oxygen-dependent degradation (ODD) domain of HIF1α responsible for the negative regulation of HIF1α in normoxia is intrinsically unfolded. Here, we carried out the backbone 1H, 15N, and 13C resonance assignment of a proteolysis-resistant fragment (residues 404-477) in the HIF1α ODD domain using NMR spectroscopy. About 98% (344/352) of all the 1HN, 15N, 13Cα, 13Cβ, and 13CO resonances were unambiguously assigned. The results will be useful for further investigation of the structural and dynamic states of the HIF1α ODD domain and its interaction with binding partners.
Keyword
hypoxia-inducible factor 1αNMR spectroscopyoxygen-dependent degradation domainresonance assignment
ISSN
1016-8478
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.1007/s10059-009-0065-4
Type
Article
Appears in Collections:
Division of Biomedical Research > 1. Journal Articles
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