Induction of DNA damage in L5178Y cells treated with gold nanoparticle

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Title
Induction of DNA damage in L5178Y cells treated with gold nanoparticle
Author(s)
J S Kang; Y N Yum; J H Kim; H Song; Jin Young Jeong; Yong Taik Lim; Bong Hyun Chung; S N Park
Bibliographic Citation
Biomolecules & Therapeutics, vol. 17, no. 1, pp. 92-97
Publication Year
2009
Abstract
As nanomaterials might enter into cells and have high reactivity with intracellular structures, it is necessary to assay possible genotoxic risk of them. One of these approaches, we investigated possible genotoxic potential of gold nanoparticle (AuNP) using L5178Y cells. Four different sizes of AuNP (4, 15, 100 or 200 nm) were synthesized and the sizes and structures of AuNP were analyzed using transmission electron microscopy (TEM), scanning electron microscopy (SEM) and stability was analyzed by a UV/Vis. Spectrophotometer. Cytotoxicity was assessed by direct cell counting, and cellular location was detected by dark field microscope at 6, 24 and 48 h after treatment of AuNP. Comet assay was conducted to examine DNA damage and tumor necrosis factor (TNF)-α mRNA level was assay by real-time reverse transcription polymerase chain reaction. Synthetic AuNP (4, 50, 100 and 200 nm size) had constant characteristics and stability confirmed by TEM, SEM and spectrophotometer for 10 days, respectively. Dark field microscope revealed the location of AuNP in the cytoplasm at 6, 24 and 48 h. Treatment of 4 nm AuNP induced dose and time dependent cytotoxicity, while other sizes of AuNP did not. However, Comet assay represented that treatment of 100 nm and 200 nm AuNP significantly increased DNA damage compared to vehicle control (p <0.01). Treatment of 100 nm and 200 nm AuNP significantly increased TNF-α mRNA expression compared to vehicle control (p<0.05, p<0.01, respectively). Taken together, AuNP induced DNA damage in L5178Y cell, associated with induction of oxidative stress.
Keyword
genetic toxicitygold nanoparticleL5178Y cellDNA damage
ISSN
1976-9148
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.4062/biomolther.2009.17.1.92
Type
Article
Appears in Collections:
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
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