An efficient plasmid vector for constitutive high-level expression of foreign genes in Escherichia coli = 대장균에서 외래유전자를 항시 고발현 시키기 위한 발현 벡터

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Title
An efficient plasmid vector for constitutive high-level expression of foreign genes in Escherichia coli = 대장균에서 외래유전자를 항시 고발현 시키기 위한 발현 벡터
Author(s)
Jeong Woo Seo; Won-Kyung Hong; D Rairakhwada; Pil-Soo Seo; Min-Ho Choi; K B Song; Sang Ki Rhee; Chul Ho Kim
Bibliographic Citation
Biotechnology Letters, vol. 31, no. 6, pp. 877-881
Publication Year
2009
Abstract
The levansucrase gene (lsrA) from Rahnella aquatilis was strongly expressed in a constitutive manner in Escherichia coli when cloned into a pBluescript KS-based pRL1CP plasmid vector. The native promoter upstream of lsrA and the lacZ promoter cooperatively enhanced the expression of lsrA to a level that was comparable to that of the T7 promoter, which is used in commercial pET expression vector system. A putative rho-independent transcription termination signal downstream of lsrA was crucial for gene expression. This plasmid vector also proved to be applicable for efficient expression of other foreign genes in E. coli.
Keyword
escherichia coligene expressionlevansucrase genepromoterrahnella aquatilis
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10529-009-9941-4
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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