High-level production of lycopene in metabolically engineered E. coli

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Title
High-level production of lycopene in metabolically engineered E. coli
Author(s)
S W Kim; J B Kim; J M Ryu; Joon Ki Jung; J H Kim
Bibliographic Citation
Process Biochemistry, vol. 44, no. 8, pp. 899-905
Publication Year
2009
Abstract
Recombinant lycopene was generated by utilizing metabolically engineered Escherichia coli with yields being dependent upon inocula state. Yields were especially low in the case of cultures harboring high-copy plasmids that were established with inocula at the stationary growth phase. On the other hand, cultures derived using low-copy plasmid, however, yielded high amounts of lycopene irrespective of inocula state. Nevertheless, it showed still an inocula dependence pattern in lycopene productivity (mg/l/h). To further increase lycopene productivity, we applied a temperature-shift culture technique (37 → 25 °C). Using this method, we effectively enhanced lycopene productivity without any problematic phenomena. As a result, we were able to increase lycopene yield by approximately 20% compared to previous culture methods. In the present study, we were able to reach a final lycopene yield up to 260 mg/l for 60 h, which corresponds to the highest titer to date for the production of lycopene in E. coli.
Keyword
inocula dependencelycopenepantoea ananatisplasmid copy numbersynthetic operontemperature-shift
ISSN
0032-9592
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.procbio.2009.04.018
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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