A simple technique to convert sitting-drop vapor diffusion into hanging-drop vapor diffusion by solidifying the reservoir solution with agarose = 아가로스를 이용한 새로운 결정화 방법
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Title
A simple technique to convert sitting-drop vapor diffusion into hanging-drop vapor diffusion by solidifying the reservoir solution with agarose = 아가로스를 이용한 새로운 결정화 방법
Author(s)
Tae Woong Whon; Y H Lee; Dong-Shan An; H K Song; Song-Gun Kim
Bibliographic Citation
Journal of Applied Crystallography, vol. 42, no. 5, pp. 975-976
Publication Year
2009
Abstract
A simple protocol to convert sitting-drop vapor-diffusion plating into a hanging-drop vapor-diffusion experiment in protein crystallization is reported. After making a sitting-drop plate, agarose solution was added to solidify the reservoir solution, and the plates were incubated upside down. Crystallization experiments with hen egg white lysozyme, thaumatin and glucose isomerase showed that the upside-down sitting-drop method could produce single crystals with all the benefits of the hanging-drop crystallization method.