Parthenogenetic induction of canine oocytes by electrical stimulation and Ca-EDTA

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Title
Parthenogenetic induction of canine oocytes by electrical stimulation and Ca-EDTA
Author(s)
S R Lee; J W Kim; B S Kim; D H Yoo; Y S Park; T H Lee; J H Ha; Byung Hwa Hyun; Z Y Ryoo
Bibliographic Citation
Reproduction in Domestic Animals, vol. 44, no. 5, pp. 740-744
Publication Year
2009
Abstract
Contents In this study, we investigated parthenogenetic induction of canine oocytes by electrical stimulation following Ca-EDTA treatment. Oocyte maturation, parthenogenetic development, and cleavage rate in canine after various electrical stimulations (1.5, 1.8, 2.1 kV/cm) for 50 μs with single DC pulse following 1 mM Ca-EDTA treatment were investigated. In oocyte activated electrically at the voltage of 1.5 kV/cm after 1 mM Ca-EDTA treatment, the rate of pronucleus and two-cell was 4.1% and 2.7%, respectively. Although electrical stimulation could parthenogenetically induce immature oocyte to cleavage stage, degeneration rate in all experimental groups was more than 60%. This means that electrical stimulation after Ca-EDTA treatment could cause canine oocytes to be degenerated. However, two-cell in canine oocyte by parthenogenesis was for the first time induced. Therefore, we suggested that electrical stimulation for canine oocytes could induce parthenogenetically early embryonic cleavage. This result can be used as a basic data for parthenogenesis study in canine. Also, to perform more developed embryonic development, further study to parthenogenesis in canine need to be developed.
ISSN
0936-6768
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/j.1439-0531.2008.01062.x
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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