Immunomodulatory effects of specific bacterial components of Lactobacillus plantarum KFCC11389P on the murine macrophage cell line RAW 264.7

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Title
Immunomodulatory effects of specific bacterial components of Lactobacillus plantarum KFCC11389P on the murine macrophage cell line RAW 264.7
Author(s)
H Chon; B Choi; Eun Gyo Lee; Seung-hui Lee; G Jeong
Bibliographic Citation
Journal of Applied Microbiology, vol. 107, no. 5, pp. 1588-1597
Publication Year
2009
Abstract
Aims: The objective of this study was to investigate the ability of specific bacterial components of Lactobacillus plantarum KFCC11389P to induce anti-inflammatory mediators in cell cultures of the murine macrophage cell line, RAW 264·7. Methods and Results: The RAW 264·7 cells were stimulated with viable bacterial cells (VC), heat-killed (HK) cells, cell walls (CW) or ultrafiltrates of metabolic products (UF). An increase in the levels of tumour necrosis factor (TNF)-α was observed in VC, HK and CW, but this effect was much lower in UF. VC stimulated higher levels of interleukin (IL)-6 releases as well as nitric oxide production than HK. In contrast, UF and its separated molecule, fraction 4, were much strong IL-10 inducers. Fraction 4 (8·1 kDa), especially, inhibited the production of pro-inflammatory cytokines, IL-6 (89% decrease) and TNF-α (55% decrease), in lipopolysaccharide (LPS)-stimulated murine macrophages. Conclusions: The results of this study indicate that metabolic products of Lact. plantarum KFCC11389P could influence the immune-modulating activity via IL-10, and pretreatment with this specific molecule could inhibit LPS-induced release of IL-6 and TNF-α. Significance and Impact of the Study: Our findings suggest that the specific molecules of Lact. plantarum KFCC11389P may be useful for the treatment of acute inflammatory responses such as Crohn's disease or ulcerative colitis.
Keyword
Anti-inflammationInterleukin-10Lactic acid bacteriaRAW 264·7 cells
ISSN
1364-5072
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/j.1365-2672.2009.04343.x
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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