Production of lactosucrose from sucrose and lactose by a levansucrase from Zymomonas mobilis = Zymomonas mobilis 유래 레반슈크라제를 이용한 락토슈크로스 생산

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Title
Production of lactosucrose from sucrose and lactose by a levansucrase from Zymomonas mobilis = Zymomonas mobilis 유래 레반슈크라제를 이용한 락토슈크로스 생산
Author(s)
W C Han; S H Byun; M H Kim; E H Sohn; J D Lim; B H Um; Chul Ho Kim; S A Kang; K H Jang
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 19, no. 10, pp. 1153-1160
Publication Year
2009
Abstract
Lactosucrose (4G-β-D-galactosylsucrose) is an oligosaccharide consisting of galactose, glucose, and fructose. In this study, we prepared lactosucrose from lactose and sucrose using a levansucrase derived from Zymomonas mobilis. Optimum conditions for lactosucrose formation were 23°C, pH 7.0, 18.0% (w/v) lactose monohydrate, and 18% (w/v) sucrose as substrates, and 1 unit of enzyme/ml of reaction mixture. Under these conditions, the lactosucrose conversion efficiency was 28.5%. The product was purified and confirmed to be O-β-D-galactopyranosyl-(1→4)-O-β-D- glucopyranosyl-(1→2)-β-D-fructofuranoside, or lactosucrose. A mixed-enzyme system containing a levansucrase and a glucose oxidase was applied in order to increase the efficiency of lactose and sucrose conversion to lactosucrose, which rose to 43.2% as a result.
Keyword
Glucose oxidaseLactosucroseLevansucraseZymomonas mobilis
ISSN
1017-7825
Publisher
South Korea
DOI
http://dx.doi.org/10.4014/jmb.0901.045
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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