Improved ethanol tolerance in Escherichia coli by changing the cellular fatty acids composition through genetic manipulation = 세포막지방산 조성의 제어를 통한 에탄올 내성 증진

Cited 38 time in scopus
Metadata Downloads
Title
Improved ethanol tolerance in Escherichia coli by changing the cellular fatty acids composition through genetic manipulation = 세포막지방산 조성의 제어를 통한 에탄올 내성 증진
Author(s)
L H Luo; Pil-Soo Seo; Jeong Woo SeoSun Yeon Heo; D H Kim; Chul Ho Kim
Bibliographic Citation
Biotechnology Letters, vol. 31, no. 12, pp. 1867-1871
Publication Year
2009
Abstract
To investigate the effect of cellular fatty acids composition on ethanol tolerance in Escherichia coli, we overexpressed either des, encoding fatty acid desaturase from Bacillus subtilis, or fabA, encoding β-hydroxydecanoyl thio-ester dehydrase from E. coli, or both genes together, into E. coli. Recombinant E. coli harboring fabA had elevated tolerance against ethanol compared to wild type strain. In contrast, des decreased resistance to ethanol. Co-expression of both genes together complemented ethanol tolerance of E. coli. This result indicates how to engineer bacterial strains to be resistant to higher concentrations of ethanol.
Keyword
DesaturaseEscherichia coliEthanol toleranceFabAFatty acids composition
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10529-009-0092-4
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.