Gene delivery using a derivative of the protein transduction domain peptide, K-Antp

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dc.contributor.authorS H Min-
dc.contributor.authorD M Kim-
dc.contributor.authorM N Kim-
dc.contributor.authorJ Ge-
dc.contributor.authorDong Chul Lee-
dc.contributor.authorIn Young Park-
dc.contributor.authorKyung Chan Park-
dc.contributor.authorJ S Hwang-
dc.contributor.authorC W Cho-
dc.contributor.authorYoung Il Yeom-
dc.date.accessioned2017-04-19T09:17:15Z-
dc.date.available2017-04-19T09:17:15Z-
dc.date.issued2010-
dc.identifier.issn0142-9612-
dc.identifier.uri10.1016/j.biomaterials.2009.11.019ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9356-
dc.description.abstractDue to their intracellular permeability, protein transduction domains (PTDs) have been widely used to deliver proteins and peptides to mammalian cells. However, their performance in gene delivery has been relatively poor. To improve the efficiency of PTD-mediated gene delivery, we synthesized a new peptide, KALA-Antp (K-Antp), which contains the sequences for PTD of the third α-helix of Antennapedia (Antp) homeodomain and the fusogenic peptide KALA. In this configuration, Antp is designed to provide the cell permeation capacity and nuclear localization signal, while the KALA moiety to promote cellular entry of the peptide-DNA complex. An optimal K-Antp/DNA formula was nearly 400-600 fold more efficient than Antp or poly-lysine-Antp (L-Antp) in gene delivery, and comparable or superior to a commercial liposome. The K-Antp-mediated plasmid DNA transfection not only exhibited temperature sensitivity, reflecting the involvement of an endocytosis-mediated gene transfer mechanism similar to other known PTDs, but also temperature insensitivity, suggesting the role of an energy-independent mechanism. Incorporation of an endosomolytic polymer polyethylenimine (PEI) into the system or treatment with chloroquine further increased the efficiency of K-Antp-mediated gene delivery. These results demonstrate the potential of the combinatorial use of KALA, Antp and PEI in the development of efficient PTD-derived gene carriers.-
dc.publisherElsevier-
dc.titleGene delivery using a derivative of the protein transduction domain peptide, K-Antp-
dc.title.alternativeGene delivery using a derivative of the protein transduction domain peptide, K-Antp-
dc.typeArticle-
dc.citation.titleBiomaterials-
dc.citation.number7-
dc.citation.endPage1864-
dc.citation.startPage1858-
dc.citation.volume31-
dc.contributor.affiliatedAuthorDong Chul Lee-
dc.contributor.affiliatedAuthorIn Young Park-
dc.contributor.affiliatedAuthorKyung Chan Park-
dc.contributor.affiliatedAuthorYoung Il Yeom-
dc.contributor.alternativeName민상현-
dc.contributor.alternativeName김동민-
dc.contributor.alternativeName김미나-
dc.contributor.alternativeNameGe-
dc.contributor.alternativeName이동철-
dc.contributor.alternativeName박인영-
dc.contributor.alternativeName박경찬-
dc.contributor.alternativeName황지숙-
dc.contributor.alternativeName조청원-
dc.contributor.alternativeName염영일-
dc.identifier.bibliographicCitationBiomaterials, vol. 31, no. 7, pp. 1858-1864-
dc.identifier.doi10.1016/j.biomaterials.2009.11.019-
dc.subject.keywordAntennapedia peptide (Antp)-
dc.subject.keywordFusogenic peptide-
dc.subject.keywordGene delivery-
dc.subject.keywordKALA-
dc.subject.keywordKALA-Antp (K-Antp)-
dc.subject.keywordProtein transduction domain (PTD)-
dc.subject.localAntennapedia peptide (Antp)-
dc.subject.localFusogenic peptide-
dc.subject.localgene delivery-
dc.subject.localGene delivery-
dc.subject.localKALA-
dc.subject.localKALA-Antp (K-Antp)-
dc.subject.localProtein transduction domain-
dc.subject.localProtein transduction domain (PTD)-
dc.description.journalClassY-
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Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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