Application of rpoB and Zinc Protease gene for use in molecular discrimination of Fusobacterium nucleatum subspecies

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Title
Application of rpoB and Zinc Protease gene for use in molecular discrimination of Fusobacterium nucleatum subspecies
Author(s)
H S Kim; Dae Sil Lee; Young Hyo Chang; M J Kim; S Koh; Joong Su Kim; J H Seong; S K Song; H S Shin; J B Son; Min-Young Jung; S N Park; S Y Yoo; K W Cho; D K Kim; Seong Hoon Moon; D Kim; Y Choi; B O Kim; H S Jang; C S Kim; C Kim; S J Choe; J K Kook
Bibliographic Citation
Journal of Clinical Microbiology, vol. 48, no. 2, pp. 545-553
Publication Year
2010
Abstract
Fusobacterium nucleatum is classified into five subspecies that inhabit the human oral cavity (F. nucleatum subsp. nucleatum, F. nucleatum subsp. polymorphum, F. nucleatum subsp. fusiforme, F. nucleatum subsp. vincentii, and F. nucleatum subsp. animalis) based on several phenotypic characteristics and DNA-DNA hybridization patterns. However, the methods for detecting or discriminating the clinical isolates of F. nucleatum at the subspecies levels are laborious, expensive, and time-consuming. Therefore, in this study, the nucleotide sequences of the RNA polymerase β-subunit gene (rpoB) and zinc protease gene were analyzed to discriminate the subspecies of F. nucleatum. The partial sequences of rpoB (approximately 2,419 bp), the zinc protease gene (878 bp), and 16S rRNA genes (approximately 1,500 bp) of the type strains of five subspecies, 28 clinical isolates of F. nucleatum, and 10 strains of F. periodonticum (as a control group) were determined and analyzed. The phylogenetic data showed that the rpoB and zinc protease gene sequences clearly delineated the subspecies of F. nucleatum and provided higher resolution than the 16S rRNA gene sequences in this respect. According to the phylogenetic analysis of rpoB and the zinc protease gene, F. nucleatum subsp. vincentii and F. nucleatum subsp. fusiforme might be classified into a single subspecies. Five clinical isolates could be delineated as a new subspecies of F. nucleatum. The results suggest that rpoB and the zinc protease gene are efficient targets for the discrimination and taxonomic analysis of the subspecies of F. nucleatum.
ISSN
0095-1137
Publisher
Amer Soc Microb
DOI
http://dx.doi.org/10.1128/JCM.01631-09
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > ABS Research Support Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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