Four different ways of alternative transcripts generation mechanism in ADRA1A gene = ADRA1A 유전자의 4개의 alternative 전사체의 생성 기작

Cited 3 time in scopus
Metadata Downloads
Title
Four different ways of alternative transcripts generation mechanism in ADRA1A gene = ADRA1A 유전자의 4개의 alternative 전사체의 생성 기작
Author(s)
Jae Won HuhYoung-Hyun KimSang Rae LeeDae Soo KimSang Je Park; H Kim; Ji Su KimBong Seok Song; H S Kim; Kyu Tae Chang
Bibliographic Citation
Genes & Genetic Systems, vol. 85, no. 1, pp. 65-73
Publication Year
2010
Abstract
The ADRA1A (Alpha-1-adrenergic receptor) gene is one of the members of G protein-coupled receptor superfamily. Alternative splicing of this gene was known to generate four transcript variants which code four isoforms with various C-terminal regions. In this study, we conducted expression analysis and evolu- tionary characterization of alternative transcripts of the ADRA1A gene. In total, 10 alternative transcripts were identified using experimental approaches and in silico analysis. Among them, 6 alternative transcripts (T1, T2, T3, T4, T4-1, and T8) were validated by RT-PCR approaches and sequencing procedures. From the alternative splicing analysis, it could be assumed that there were three different alternative transcripts generation mechanisms and unknown mechanism. First one is the integration event of three different TEs (AluSc, L1MC5, and MIR3) as seen on the last exons of T3, T4, T4-1, T5, T6, and T7 transcripts. The second mechanism is a differential promoter usage on T8. The third one is a substitution event of the 3' splicing site during the primate evolution on T3. The last one is an unknown mechanism which was identified in the T4-1 transcript. Therefore, alternative transcripts of human ADRA1A gene occurred by four different ways, such as integration of TEs, differential promoter usage, substitution of splicing sites, and unknown mechanism.
Keyword
Adra1a geneAlternative splicingSubstitutionTes integration
ISSN
1341-7568
Publisher
Genetics Soc Japan
DOI
http://dx.doi.org/10.1266/ggs.85.65
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > National Primate Research Center > 1. Journal Articles
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.