A highly sensitive enzyme-amplified immunosensor based on a nanoporous niobium oxide (Nb2O5) electrode = 다공성 니오븀산화물 전극을 이용한 고감도 효소증폭 면역센서

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Title
A highly sensitive enzyme-amplified immunosensor based on a nanoporous niobium oxide (Nb2O5) electrode = 다공성 니오븀산화물 전극을 이용한 고감도 효소증폭 면역센서
Author(s)
Chang-Soo Lee; Dohyoung Kwon; J E Yoo; B G Lee; J Choi; Bong Hyun Chung
Bibliographic Citation
Sensors, vol. 10, no. 5, pp. 5160-5170
Publication Year
2010
Abstract
We report on the development of an enzyme-amplified sandwich-type immunosensor based on a thin gold film sputtered on an anodic nanoporous niobium oxide (Au@Nb2O5) electrode. The electrocatalytic activity of enzymatically amplified electroactive species and a stable electrode consisting of Au@Nb2O5 were used to obtain a powerful signal amplification of the electrochemical immunobiosensor. The method using this electrochemical biosensor based on an Au@Nb2O5 electrode provides a much better performance than those based on conventional bulk gold or niobium oxide electrodes. Our novel approach does not require any time-consuming cleaning steps to yield reproducible electrochemical signals. In addition, the strong adhesion of gold films on the niobium oxide electrodes offers a very stable substrate during electrochemical biosensing. Cyclic voltammetry measurements indicate that non-specific binding of proteins to the modified Au@Nb2O5 surface is sufficiently low to be ignored in the case of our novel system. Finally, we demonstrated the ability of the biosensor based on an Au@Nb2O5 offering the enhanced performance with a high resolution and sensitivity. Therefore, it is expected that the biosensor based on an Au@Nb2O5 has great potential for highly efficient biological devices.
Keyword
Electrochemical biosensorEnzymeImmunosensorNiobium oxide
ISSN
1424-8220
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/s100505160
Type
Article
Appears in Collections:
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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