Cloning, expression and characterization of xylose isomerase, XylA, from Caldanaerobacter subterraneus subsp. yonseiensis

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Title
Cloning, expression and characterization of xylose isomerase, XylA, from Caldanaerobacter subterraneus subsp. yonseiensis
Author(s)
Byoung Chan Kim; S N Yu; K Y Kim; Jung-Sook Lee; Y R Pyun; S C Ahn
Bibliographic Citation
Biotechnology Letters, vol. 32, no. 7, pp. 929-933
Publication Year
2010
Abstract
The xylA gene, coding for xylose isomerase, from the extreme thermophile, Caldanaerobacter subterraneus subsp. yonseiensis was cloned, sequenced, and expressed in Escherichia coli. The nucleotide sequence of the xylA gene encoded a polypeptide of 438 residues with a calculated molecular weight of 50,170 Da. The purified XylA showed high sequence homology (92% identity) with that of Thermoanaerobacter thermohydrosulfuricus. The recombinant enzyme expressed in Escherichia coli was purified by heat treatment and gel chromatography. The purified enzyme was thermostable with optimal activity at 95°C. The enzyme required divalent cations including Zn2+ for its maximal activity and thermostability.
Keyword
Caldanaerobacter subterraneusThermoanaerobacterThermophileXylAXylose isomerase
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10529-010-0255-3
Type
Article
Appears in Collections:
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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