Enhanced production of ethanol from glycerol by engineered Hansenula polymorpha expressing pyruvate decarboxylase and aldehyde dehydrogenase genes from Zymomonas mobilis = 재조합효모를 이용한 글리세롤로부터 에탄올 생산
Cited 29 time in
- Enhanced production of ethanol from glycerol by engineered Hansenula polymorpha expressing pyruvate decarboxylase and aldehyde dehydrogenase genes from Zymomonas mobilis = 재조합효모를 이용한 글리세롤로부터 에탄올 생산
- Won Kyung Hong; Chul Ho Kim; Sun Yeon Heo; L H Luo; Baek Rock Oh; Jeong Woo Seo
- Bibliographic Citation
- Biotechnology Letters, vol. 32, no. 8, pp. 1077-1082
- Publication Year
- To improve production of ethanol from glycerol, the methylotrophic yeast Hansenula polymorpha was engineered to express the pdc and adhB genes encoding pyruvate decarboxylase and aldehyde dehydrogenase II from Zymomonas mobilis, respectively, under the control of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. The ethanol yield was 3.3-fold higher (2.74 g l-1) in the engineered yeast compared with the parent strain (0.83 g l-1). Further engineering to stimulate glycerol utilization in the recombinant strain via expression of dhaD and dhaKLM genes from Klebsiella pneumoniae encoding glycerol dehydrogenase and dehydroxyacetone kinase, respectively, resulted in a 3.7-fold increase (3.1 g l-1) in ethanol yield.
- Aldehyde dehydrogenase II; Ethanol production; Glycerol; Hansenula polymorpha; Pyruvate decarboxylase
- Appears in Collections:
- Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
- Files in This Item:
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.