Enhanced production of ethanol from glycerol by engineered Hansenula polymorpha expressing pyruvate decarboxylase and aldehyde dehydrogenase genes from Zymomonas mobilis = 재조합효모를 이용한 글리세롤로부터 에탄올 생산

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Title
Enhanced production of ethanol from glycerol by engineered Hansenula polymorpha expressing pyruvate decarboxylase and aldehyde dehydrogenase genes from Zymomonas mobilis = 재조합효모를 이용한 글리세롤로부터 에탄올 생산
Author(s)
Won Kyung Hong; Chul Ho KimSun Yeon Heo; L H Luo; Baek Rock OhJeong Woo Seo
Bibliographic Citation
Biotechnology Letters, vol. 32, no. 8, pp. 1077-1082
Publication Year
2010
Abstract
To improve production of ethanol from glycerol, the methylotrophic yeast Hansenula polymorpha was engineered to express the pdc and adhB genes encoding pyruvate decarboxylase and aldehyde dehydrogenase II from Zymomonas mobilis, respectively, under the control of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. The ethanol yield was 3.3-fold higher (2.74 g l-1) in the engineered yeast compared with the parent strain (0.83 g l-1). Further engineering to stimulate glycerol utilization in the recombinant strain via expression of dhaD and dhaKLM genes from Klebsiella pneumoniae encoding glycerol dehydrogenase and dehydroxyacetone kinase, respectively, resulted in a 3.7-fold increase (3.1 g l-1) in ethanol yield.
Keyword
Aldehyde dehydrogenase IIEthanol productionGlycerolHansenula polymorphaPyruvate decarboxylase
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10529-010-0259-z
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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