S. pombe genome deletion project: An update

Cited 32 time in scopus
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Title
S. pombe genome deletion project: An update
Author(s)
M Spirek; Z Benko; M Carnecka; C Rumpf; L Cipak; M Batova; I Marova; M Nam; Dong Uk Kim; H O Park; J Hayles; Kwang Lae Hoe; P Nurse; J Gregan
Bibliographic Citation
Cell Cycle, vol. 9, no. 12, pp. 2399-2402
Publication Year
2010
Abstract
The fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection of heterozygous diploid strains containing individual deletions in nearly all S. pombe genes has been created using a PCR based strategy. However, deletion of some genes has not been possible using this methodology. Here we use an efficient knockout strategy based on plasmids that contain large regions homologous to the target gene to delete an additional 29 genes. The collection of deletion mutants now covers 99% of the fission yeast open reading frames.
Keyword
Essential genesFission yeastGene deletionGenomicsVectors
ISSN
1538-4101
Publisher
T&F (Taylor & Francis)
DOI
http://dx.doi.org/10.4161/cc.9.12.11914
Type
Article
Appears in Collections:
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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