Fast microchip electrophoresis using field strength gradients for single nucleotide polymorphism identification of cattle breeds

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Title
Fast microchip electrophoresis using field strength gradients for single nucleotide polymorphism identification of cattle breeds
Author(s)
D Oh; I C Cheong; Hee Gu Lee; S K Eo; S H Kang
Bibliographic Citation
Bulletin of Korean Chemical Society, vol. 31, no. 7, pp. 1902-1906
Publication Year
2010
Abstract
A microchip electrophoresis (ME) method was developed using a programmed field strength gradients (PFSG) for the single nucleotide polymorphism (SNP) based fast identification of cattle breeds. Four different Korean cattle (Hanwoo) and Holstein SNP markers amplified by allele-specific polymerase chain reaction were separated in a glass microchip filled with 0.5% poly(ethyleneoxide) (Mr = 8 000 000) by PFSG as follows: 750 V/cm for 0 - 14 s, 166.7 V/cm for 14 - 31 s, 83.3 V/cm for 31 - 46 s, and 750 V/cm for 46 - 100 s. The cattle breeds were clearly distinguished within 45 s. The ME-PFSG method was 7 times and 5 times faster than the constant electric field ME method and the capillary electrophoresis- PFSG method, respectively, with a high resolving power (Rs = 5.05 - 9.98). The proposed methodology could be a powerful tool for the fast and simultaneous determination of SNP markers for various cattle breeds with high accuracy.
Keyword
Cattle breedFast separationField strength gradientsMicrochip gel electrophoresisSingle nucleotide polymorphism (SNP)
ISSN
0253-2964
Publisher
Wiley
DOI
http://dx.doi.org/10.5012/bkcs.2010.31.7.1902
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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