Diversity and abundance of ammonia-oxidizing bacteria in activated sludge treating different types of wastewater

Cited 22 time in scopus
Metadata Downloads
Title
Diversity and abundance of ammonia-oxidizing bacteria in activated sludge treating different types of wastewater
Author(s)
K Baek; C Park; Hee-Mock Oh; Byung Dae Yoon; Hee-Sik Kim
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 20, no. 7, pp. 1128-1133
Publication Year
2010
Abstract
The diversity and abundance of ammonia-oxidizing bacteria (AOB) in activated sludge were compared using PCR- DGGE and real-time PCR assays. Activated sludge samples were collected from five different types of wastewater treatment plants (WWTPs) mainly treating textile, paper, food, and livestock wastewater or domestic sewage. The composition of total bacteria determined by PCR-DGGE was highly diverse between the samples, whereas the community of AOB was similar across all the investigated activated sludge. Total bacterial numbers and AOB numbers in the aerated mixed liquor were in the range of 1.8x1010 to 3.8x1012 and 1.7x106 to 2.7x1010 copies/l, respectively. Activated sludge from livestock, textile, and sewage treating WWTPs contained relatively high amoA gene copies (more than 105 copies/l), whereas activated sludge from food and paper WWTPs revealed a low number of the amoA gene (less than 103 copies/l). The value of the amoA gene copy effectively showed the difference in composition of bacteria in different activated sludge samples and this was better than the measurement with the AOB 16S rRNA or total 16S rRNA gene. These results suggest that the quantification of the amoA gene can help monitor AOB and ammonia oxidation in WWTPs.
Keyword
Activated sludgeAmmonia-oxidizing bacteriaPCR-DGGEReal-time PCRWastewater
ISSN
1017-7825
Publisher
South Korea
DOI
http://dx.doi.org/10.4014/jmb.0907.07021
Type
Article
Appears in Collections:
Division of Biomaterials Research > Cell Factory Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.