Isolation of a calcium-binding substance from anchovy

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Title
Isolation of a calcium-binding substance from anchovy
Author(s)
S J Jeon; Won Gon Kim; K B Song
Bibliographic Citation
Journal of Korean Society for Applied Biological Chemistry, vol. 53, no. 4, pp. 516-518
Publication Year
2010
Abstract
Dried anchovy (Engraulis japonica) was extracted using hot water and filtered with a YM-3 membrane to isolate a calcium binding substance. The membrane filtered solution was fractionated using gel permeation chromatography, ion exchange chromatography, reverse-phase high performance liquid chromatography, and fast protein liquid chromatography to detect calcium-binding substances. The isolated substance was identified as adenosine by nuclear magnetic resonance spectroscopy.
Keyword
AnchovyCalcium-binding substancePurification
ISSN
I000-0171
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.3839/jksabc.2010.079
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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