Random breakup of microdroplets for single-cell encapsulation

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Title
Random breakup of microdroplets for single-cell encapsulation
Author(s)
E Um; Seung Goo Lee; J K Park
Bibliographic Citation
Applied Physics Letters, vol. 97, no. 15, pp. 153703-153703
Publication Year
2010
Abstract
Microfluidic droplet-based technology enables encapsulation of cells in the isolated aqueous chambers surrounded by immiscible fluid but single-cell encapsulation efficiency is usually less than 30%. In this letter, we introduce a simple microgroove structure to break droplets into random sizes which further allows collecting of single-cell [Escherichia coli (E. coli)] containing droplets by their size differences. Pinched-flow separation method is integrated to sort out droplets of certain sizes which have high probability of containing one cell. Consequently, we were able to obtain more than 50% of droplets having single E. coli inside, keeping the proportion of multiple-cell containing droplets less than 16%.
ISSN
0003-6951
Publisher
Amer Inst Phys
DOI
http://dx.doi.org/10.1063/1.3500980
Type
Article
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
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