In vitro anti-rotavirus activity of polyphenol compounds isolated from the roots of Glycyrrhiza uralensis = 감초로부터 항로타바이러스활성을 지니는 폴리페놀화합물의 개발

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Title
In vitro anti-rotavirus activity of polyphenol compounds isolated from the roots of Glycyrrhiza uralensis = 감초로부터 항로타바이러스활성을 지니는 폴리페놀화합물의 개발
Author(s)
Hyung Jun Kwon; Ha-Hyeon Kim; Young Bae Ryu; Jang Hoon Kim; Hyung Jae JungSeung Woong Lee; Jong Sun Chang; K O Cho; Mun Chual RhoSu-Jin ParkWoo Song Lee
Bibliographic Citation
Bioorganic & Medicinal Chemistry, vol. 18, no. 21, pp. 7668-7674
Publication Year
2010
Abstract
We evaluated the ability of six polyphenols isolated from the roots of Glycyrrhiza uralensis to inactivate rotaviruses, specially G5P[7] and G8P[7]. Upon finding that all polyphenols possessed anti-rotavirus activity, we evaluated whether these properties were attributable to direct inhibition of the binding of rotavirus to cells and/or to inhibition of viral replication. Using the virucidal assay, we found that all six compounds directly inhibited rotavirus binding, with activity being dependent on the type of virus. The 50% effective inhibitory concentrations (EC50) of the six compounds were 18.7-69.5 μM against G5P[7] and 14.7-88.1 μM against G8P[7], respectively. Five of the six compounds inhibited hemagglutination activity. Moreover, the CPE inhibition assay showed that five compounds inhibited viral replication with EC50 values of 12.1-24.0 μM against G5P[7] and 12.0-42.0 μM against G8P[7], respectively. RT-PCR showed that the compounds suppressed viral RNA synthesis in TF-104 cells. Interestingly, the anti-rotavirus activities of four compounds were attributable to inhibition of both viral absorption and viral replication. These results suggest that compounds isolated from the roots of G. uralensis may be potent anti-rotavirus agents in vivo, acting by inhibiting both viral absorption and viral replication.
Keyword
Anti-rotavirusGlycyrrhiza uralensisHemagglutininViral absorptionViral replication
ISSN
0968-0896
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bmc.2010.07.073
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Immunoregulatory materials Research Center > 1. Journal Articles
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