Evaluation of NPTII gene transfer from transgenic chili pepper to culturable microorganisms in rhizosphere soil = 형질전환 고추로부터 근권 토양 미생물로의 수평유전자이동 조사

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Title
Evaluation of NPTII gene transfer from transgenic chili pepper to culturable microorganisms in rhizosphere soil = 형질전환 고추로부터 근권 토양 미생물로의 수평유전자이동 조사
Author(s)
B Lee; J Y Park; Kee Woong Park; C H Harn; Hwan Mook Kim; Chang-Gi Kim
Bibliographic Citation
Korean Journal of International Agriculture, vol. 22, no. 3, pp. 288-292
Publication Year
2010
Abstract
The possible gene transfer from transgenic chili pepper to soil bacteria in rhizosphere was evaluated. DNA was extracted from rhizosphere soil collected from growing transgenic chili pepper (Capsicum annuum L.) resistant to cucumber mosaic virus (CMV) and control line, and PCR analysis was performed with specific primer for transgene in the transgenic chili pepper. The results of the PCR targeting NPTII gene indicated that the transgene was present in the rhizosphere soil samples in all collecting times. To investigate the cause of the transgene detection in the samples, we isolated microorganisms from the soil and cultured them on bacterial and fungal medium containing kanamycin. About 430 thousands of bacterial colonies and 160 thousands of fungal colonies were investigated, however, colonies resistant to kanamycin were not detected, indicating gene transfer from transgenic chili pepper to culturable rhizosphere soil microorganisms was not occurred in the present study.
Keyword
Capsicum annuumchili peppergene transfermicroorganismsrhizospheretransgenic crop
ISSN
1225-8504
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > Bio-Evaluation Center > 1. Journal Articles
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