Mechanism of anguillosporal inhibitory activity for phospholipase C as the signal transducer in the biological networking = 생체내 정보 전달계 효소인 phospholipase C 활성을 저해하는 anguillosporal의 작용기전

Abstract

Anguillosporal dose-dependently inhibited phospholipase C (PLC)γ1 activity in A431 cells in response to epidermal growth factor (EGF). The compound also inhibited PLCβ1 activity in α-thrombin- stimulated CCL39 cells. In accordance with this, PLCγ1 and PLCβ1 activities were also decreased by anguillosporal in vitro. However, anguillosporal did not reduce the auto- phosphorylation of EGFR and the tyrosine phosphorylation of PLCγ1 in EGF-stimulated A431 cells. Also anguillosporal did not inhibit the tyrosine kinase activity of EGFR. From these results, it is suggested that anguillosporal is a specific inhibitor of phospholipase C.