Expression profiling of calcium induced genes in cultured human keratinocytes

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Title
Expression profiling of calcium induced genes in cultured human keratinocytes
Author(s)
J S Lee; Mirang KimNam-Soon Kim; Yong Sung Kim; J M Yang; A Y Cho; Y Lee; C D Kim; J H Lee
Bibliographic Citation
Journal of Korean Medical Science, vol. 25, no. 4, pp. 619-625
Publication Year
2010
Abstract
Terminal differentiation of skin keratinocytes is a vertically directed multi-step process that is tightly controlled by the sequential expression of a variety of genes. To examine the gene expression profile in calcium-induced keratinocyte differentiation, we constructed a normalized cDNA library using mRNA isolated from these calcium-treated keratinocytes. After sequencing about 10,000 clones, we were able to obtain 4,104 independent genes. They consisted of 3,699 annotated genes and 405 expressed sequence tags (ESTs). Some were the genes involved in constituting epidermal structures and others were unknown genes that are probably associated with keratinocytes. In particular, we were able to identify genes located at the chromosome 1q21, the locus for the epidermal differentiation complex, and 19q13.1, another probable locus for epidermal differentiation-related gene clusters. One EST located at the chromosome 19q13.1 showed increased expression by calcium treatment, suggesting a novel candidate gene relevant to keratinocyte differentiation. These results demonstrate the complexity of the transcriptional profile of keratinocytes, providing important clues on which to base further investigations of the molecular events underlying keratinocyte differentiation.
Keyword
CalciumGene expression profilingKeratinocyte differentiationNormalized library
ISSN
1011-8934
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.3346/jkms.2010.25.4.619
Type
Article
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Division of Biomedical Research > Genome Editing Research Center > 1. Journal Articles
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