Acetate accumulation through alternative metabolic pathways in ackA- pta- poxB- triple mutant in E. coli B (BL21)

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dc.contributor.authorJ N Phue-
dc.contributor.authorSang Jun Lee-
dc.contributor.authorJ B Kaufman-
dc.contributor.authorA Negrete-
dc.contributor.authorJ Shiloach-
dc.date.accessioned2017-04-19T09:20:27Z-
dc.date.available2017-04-19T09:20:27Z-
dc.date.issued2010-
dc.identifier.issn0141-5492-
dc.identifier.uri10.1007/s10529-010-0369-7ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9845-
dc.description.abstractIndividual deletions of acs and aceA genes in E. coli B (BL21) showed little difference in the metabolite accumulation patterns but deletion of the ackA gene alone or together with pta showed acetic acid gradually accumulated to 3. 1 and 1. 7 g/l, respectively, with a minimal extended lag in bacterial growth and a higher pyruvate formation. Single poxB deletion in E. coli B (BL21) or additional poxB deletion in the ackA-pta mutants did not change the acetate accumulation pattern. When the acetate production genes (ackA-pta-poxB) were deleted in E. coli B (BL21) acetate still accumulated. This may be an indication that perhaps acetate is not only a by-product of carbon metabolism; it is possible that acetate plays also a role in other cellular metabolite pathways. It is likely that there are alternative acetate production pathways.-
dc.publisherSpringer-
dc.titleAcetate accumulation through alternative metabolic pathways in ackA- pta- poxB- triple mutant in E. coli B (BL21)-
dc.title.alternativeAcetate accumulation through alternative metabolic pathways in ackA- pta- poxB- triple mutant in E. coli B (BL21)-
dc.typeArticle-
dc.citation.titleBiotechnology Letters-
dc.citation.number12-
dc.citation.endPage1903-
dc.citation.startPage1897-
dc.citation.volume32-
dc.contributor.affiliatedAuthorSang Jun Lee-
dc.contributor.alternativeNamePhue-
dc.contributor.alternativeName이상준-
dc.contributor.alternativeNameKaufman-
dc.contributor.alternativeNameNegrete-
dc.contributor.alternativeNameShiloach-
dc.identifier.bibliographicCitationBiotechnology Letters, vol. 32, no. 12, pp. 1897-1903-
dc.identifier.doi10.1007/s10529-010-0369-7-
dc.subject.keywordAcetate excretion-
dc.subject.keywordE. coli B (BL21) mutant-
dc.subject.keywordHigh glucose-
dc.subject.localAcetate excretion-
dc.subject.localE. coli B (BL21) mutant-
dc.subject.localHigh glucose-
dc.description.journalClassY-
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