Acetate accumulation through alternative metabolic pathways in ackA- pta- poxB- triple mutant in E. coli B (BL21)

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Title
Acetate accumulation through alternative metabolic pathways in ackA- pta- poxB- triple mutant in E. coli B (BL21)
Author(s)
J N Phue; Sang Jun Lee; J B Kaufman; A Negrete; J Shiloach
Bibliographic Citation
Biotechnology Letters, vol. 32, no. 12, pp. 1897-1903
Publication Year
2010
Abstract
Individual deletions of acs and aceA genes in E. coli B (BL21) showed little difference in the metabolite accumulation patterns but deletion of the ackA gene alone or together with pta showed acetic acid gradually accumulated to 3. 1 and 1. 7 g/l, respectively, with a minimal extended lag in bacterial growth and a higher pyruvate formation. Single poxB deletion in E. coli B (BL21) or additional poxB deletion in the ackA-pta mutants did not change the acetate accumulation pattern. When the acetate production genes (ackA-pta-poxB) were deleted in E. coli B (BL21) acetate still accumulated. This may be an indication that perhaps acetate is not only a by-product of carbon metabolism; it is possible that acetate plays also a role in other cellular metabolite pathways. It is likely that there are alternative acetate production pathways.
Keyword
Acetate excretionE. coli B (BL21) mutantHigh glucose
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10529-010-0369-7
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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