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- Title
- In vivo effects of NbSiR silencing on chloroplast development in Nicotiana benthamiana
- Author(s)
- Y W Kang; J Y Lee; Y Jeon; G W Cheong; Moonil Kim; H S Pai
- Bibliographic Citation
- Plant Molecular Biology, vol. 72, no. 6, pp. 569-583
- Publication Year
- 2010
- Abstract
- Sulfite reductase (SiR) performs dual functions, acting as a sulfur assimilation enzyme and as a chloroplast (cp-) nucleoid binding protein. In this study, we examined the in vivo effects of SiR deficiency on chloroplast development in Nicotiana benthamiana. Virus-induced gene silencing of NbSiR resulted in leaf yellowing and growth retardation phenotypes, which were not rescued by cysteine supplementation. NbSiR:GFP fusion protein was targeted to chloroplasts and colocalized with cp-nucleoids. Recombinant full-length NbSiR protein and the C-terminal half of NbSiR possessed cp-DNA compaction activities in vitro, and expression of full-length NbSiR in E. coli caused condensation of genomic DNA. NbSiR silencing differentially affected expression of plastid-encoded genes, inhibiting expression of several genes more severely than others. In the later stages, depletion of NbSiR resulted in chloroplast ablation. In NbSiR-silenced plants, enlarged cp-nucleoids containing an increased amount of cp-DNA were observed in the middle of the abnormal chloroplasts, and the cp-DNAs were predominantly of subgenomic sizes based on pulse field gel electrophoresis. The abnormal chloroplasts developed prolamellar body-like cubic lipid structures in the light without accumulating NADPH:protochlorophyllide oxidoreductase proteins. Our results suggest that NbSiR plays a role in cp-nucleoid metabolism, plastid gene expression, and thylakoid membrane development.
- Keyword
- Chloroplast nucleoidsDNA condensationPlastid transcriptionProlamellar bodyPulse field gel electrophoresisVirus-induced gene silencing
- ISSN
- 0167-4412
- Publisher
- Springer
- DOI
- http://dx.doi.org/10.1007/s11103-009-9593-8
- Type
- Article
- Appears in Collections:
- Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
- Files in This Item:
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