Qualitative and quantitative detection of agricultural microorganisms expressing iturin and mop cyclase in soils

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Title
Qualitative and quantitative detection of agricultural microorganisms expressing iturin and mop cyclase in soils
Author(s)
Sung Eun Kim; Jae Sun Moon; Won Sik Choi; E N Lee; S H Lee; Sung Uk Kim
Bibliographic Citation
Journal of Agricultural and Food Chemistry, vol. 58, no. 24, pp. 12657-12663
Publication Year
2010
Abstract
The environmental release of genetically engineered microorganisms (GEMs) to improve agriculture or remediate environmental hazards has raised concern over the fate of the organisms and their engineered genes. To detect the microorganisms released into the environment at the molecular level, Bacillus subtilis KB producing iturin and Pseudomonas fluorescens MX1 carrying the moc (mannityl opine catabolism) region from the Agrobacterium tumefaciens were employed as model microorganisms. Using specific fusion primers and the TaqMan probes, qualitative and quantitative detections of the model organisms by PCR and real-time PCR were conducted employing a small-scale soil-core device and pots during the six month period. The data indicate that the model bacteria can be easily detected by qualitative and quantitative methods in the test systems employed, and they do not give significant impacts on the other bacteria in soils on the Southern blotting analysis, although long-term observation may be needed. ⓒ 2010 American Chemical Society.
Keyword
Agricultural microorganismgenetically modified organism (GMO)iturinmop cyclasePGPRreal-time PCRsoil-core devicehorizontal gene transfer
ISSN
0021-8561
Publisher
Amer Chem Soc
DOI
http://dx.doi.org/10.1021/jf103216g
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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